Comparison of Methods to Generate Vialde Goat Intestinal Epithelial Call by Enzymes

To find the optimal method to isolate and culture the goat intestine epithelial cells,collagenaseⅠ、thermolysin、collagenaseⅪ/dispaseⅠdigestion technique were used to isolate IEC from the fetal goat.Optimal culture conditions were determined by both cellular morphology under light microscope and immunocytochemical characterization.The results showed that large amount of villi crypt units were attainable and the attached cells were positive for cytokeratin using collagenaseⅪ/dispaseⅠdigestion technique while many fibroblast cells contaminated when using collagenaseⅠand thermolysin digestion techniques.This study showed that viable epithelial cells can be relatively easily generated from fetal goat small intestine by collagenaseⅪ/dispaseⅠdigestion technique.