Effect and mechanism of PUMA on the apoptosis of mouse liver cell in vitro

AIM:To study the influence on the biological function of BNL.CL2 such as cell cycle and apoptosis and molecule mechanism of PUMA.METHODS:① The packaging and preparation of adenoviruses expressing PUMA(Ad-PUMA) and adenoviruses expressing GFP(Ad-GFP).② The cellular biological function of BNL CL2 cells after infected by Ad-PUMA.③ Molecular mechanisms of the effection of PUMA on mouse liver cells.RESULTS:The results indicated that the packaging and preparation of Adenoviruses expressing PUMA(Ad-PUMA) was available and effective expression in mouse liver cell BNL CL2.PUMA could induce apoptosis in these cells and the number of cells at S stage displayed increasing tendency with the infection time.Taking into account above factors,we then analyzed that PUMA induction affects the interactions between Bax and Bcl-XL,between PUMA and Bcl-XL,as well as the interactions between Bax and PUMA by immunoprecipitation.CONCLUSION:PUMA could induce apoptosis in these cells and the number of cells at S stage displayed increasing tendency with the infection time.PUMA dissociates Bax and Bcl-XL to induce apoptosis and the interact with Bax directly.