This study was to detect flavonoids in Diospyros kaki leaves by HPLC and discuss their accumulation therein, in order to find the appropriate time for harvesting Diospyros kaki leaves and determine leaf flavonoids content in different Diospyros kaki varieties in Hunan.The results are as follows: The HPLC analysis method is stable, reliable and of good repeatability; the appropriate time for harvesting Diospyros kaki leaves is in March and September and October in accordance with the leaf flavonoids content metabolism and accumulation;and there are some differences in leaf flavonoids content among 17 Diospyros kaki varieties in 7 prefectures of the province, the highest content in Ci Lang Persimmon and the lowest in Yao Dai Shi Crude persimmon.