海绵
硫酸铵沉淀
生物化学
化学
细菌
无花果素
凝集素
亲和层析
氨基酸
离子色谱法
色谱法
大小排阻色谱法
肽序列
生物
甘露聚糖结合凝集素
酶
基因
植物
遗传学
作者
Bertha Fenton,Roberto Arreguín Espinosa,Edgar Vázquez Contreras,Barbarin Arreguín Lozano,Norma Elba Sánchez,Enrique Rico Hernández,Edgar Zenteno Galindo
摘要
The purification, structural and functional characterization of two different lectins (named Svl-1 and Svl-2) has been reported from the marine sponge Spheciospongia vesparia. Purification procedure includes ammonium sulfate precipitation, combined with chromatography including Octyl-Sepharose-(NH4)SO4 hydrophobic column and DEAE-Toyopearl anion-exchange column using a high performance liquid chromatography. The similarities in function, specificity for saccharides, molecular weight, amino acid content and the N-terminal sequence of two lectins suggest that these proteins are isolectins. Amino acid composition and fluorescence analyses reveal that they contain an intrachain disulfide bridge, which might contribute to their high thermal stability. Furthermore, the purified lectins exhibit antibacterial activity against the gram-negative bacteria Pseudomonas aeruginosa and E. coli, indicating that they may be involved in a recognition strategy and may play a role in the defense response function of the sponge. This is the first report on the isolation of lectins from the S. vesparia. The purified lectins represent a potential possible candidate for future application in the recognition or treatment of cancer cells.
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