Detection of Salmonella in water bodies by LAMP rapid method

Objective A loop-mediated isothermal amplification(LAMP) method was established as substitute for time-consuming traditional detection of Salmonella spp.in environmental water body.Methods Primers for detecting Salmonella spp.were designed based on the phoP gene of Salmonella spp.The reaction conditions were verified at 63 ℃ for 60 min and then at 80 ℃ for 10 min,and the detection results were judged by naked eye.Results The sensitivity of the LAMP assay was found to be 3.07 fg/μl for the genome of cultivated Salmonella,and 2.39×10 cfu/ml for simulated contaminated environmental water samples.Conclusion LAMP assay is very rapid,sensitive and convenient for the detection of Salmonella spp.in environmental water,and it can be an alternative choice for the rapid detection of water-borne pathogens.