Angiotensin II Promotes Selective Uptake of High Density Lipoprotein Cholesterol Esters in Bovine Adrenal Glomerulosa and Human Adrenocortical Carcinoma Cells Through Induction of Scavenger Receptor Class B Type I

Angiotensin II is one of the main physiological regulators of aldosterone biosynthesis in the zona glomerulosa of the adrenal cortex.The hormone stimulates intracellular cholesterol mobilization to the mitochondrion for steroid biosynthesis.Here we have examined whether angiotensin II also modulates exogenous lipoprotein cholesterol ester supply to the steroidogenic machinery and whether this control is exerted on the selective transport of high density lipoproteinderived cholesterol ester to intracellular lipid droplets through the scavenger receptor class B type I.In bovine adrenal glomerulosa and human NCI H295R adrenocortical carcinoma cells, high density lipoprotein stimulated steroid production.Angiotensin II pretreatment for 24 h potentiated this response.Fluorescence microscopy of cellular uptake of reconstituted high density lipoprotein containing a fluorescent cholesterol ester revealed an initial, time-dependent narrow labeling of the cell membrane followed by an intense accumulation of the fluorescent cholesterol ester within lipid droplets.At all time points, labeling was more pronounced in cells that had been treated for 24 h with angiotensin II.Fluorescence incorporation into cells was prevented by a mono-clonal antibody directed against apolipoprotein A-I.Upon quantitative fluorometric determination, cholesterol ester uptake in angiotensin II-treated bovine cells was increased to 175 ؎ 15% of controls after 2 h and to 260 ؎ 10% after 4 h of exposure to fluorescent high density lipoprotein.The amount of scavenger receptor class B type I protein detected in cells treated with angiotensin II for 24 h reached 203 ؎ 12% of that measured in control cells (n ‫؍‬ 3, P < 0.01).In contrast, low density lipoprotein receptors were only minimally affected by angiotensin II treatment.This increase in scavenger receptor class B type I protein was associated with a 3-fold induction of scavenger receptor class B type I mRNA, which could be prevented by actinomycin D but not by cycloheximide.Similar results were obtained in the human adenocarcinoma cell line H295R.These observations show that angiotensin II regulates the scavenger receptor class B type I-mediated selective transport of lipoprotein cholesterol ester across the cell membrane as a major source of precursor for mineralocorticoid biosynthesis in both human and bovine adrenal cells.