The Calcineurin/Nuclear Factor of Activated T Cells Signaling Pathway Regulates Osteoclastogenesis in RAW264.7 Cells

Although best known for its role in T lymphocyte activation, the calcineurin/nuclear factor of activated T cells (NFAT) signaling pathway is also known to be involved in a wide range of other biological responses in a variety of different cell types. Here we have investigated the role of the calcineurin/NFAT signaling pathway in the regulation of osteoclast differentiation. Osteoclasts cells the cell a of the factor of known of of the the of a the of the cell we in cells is in to we of a in cells is to cells to of of the of a of the pathway the of osteoclast the of the factor is to a in the of the osteoclast cell Although best known for its role in T lymphocyte activation, the calcineurin/nuclear factor of activated T cells (NFAT) signaling pathway is also known to be involved in a wide range of other biological responses in a variety of different cell types. Here we have investigated the role of the calcineurin/NFAT signaling pathway in the regulation of osteoclast differentiation. Osteoclasts cells the cell a of the factor of known of of the the of a the of the cell we in cells is in to we of a in cells is to cells to of of the of a of the pathway the of osteoclast the of the factor is to a in the of the osteoclast cell is a is a of is a the of the cells the cell is for in the signaling osteoclast have the cells cells the is osteoclast is the of cells is a wide range of a of a of the factor of known of cell activated factor of T of factor cell activated factor of T of factor also known is cells role in the regulation of osteoclast a of is to the for cell in the of osteoclast in in known to have to the of cells its a of the factor is osteoclast of the in the regulation of is the in a of a in osteoclast is known to a of factor factor signaling of a of signaling the in have the of a of the factor for osteoclast in is for to in have for the in the regulation of osteoclast signaling is for osteoclast of the of signaling involved in is have the have the of to in to a variety of different also to in osteoclast Although to in the regulation of osteoclast activation, is the osteoclast is the for the of is the osteoclast the is to the of the of a in the regulation of is of the is best known for the role T cell activation, to the of the factor of activated T cells (NFAT) of of the T cell to in the of other T is activated in to the T cell in the in a in the the in the factor to to the of the in of to the of the of a other known to role in the regulation of the Although the calcineurin/NFAT signaling pathway is best known for its role in the regulation of the pathway is also in a wide range of cell of the a role in the regulation of cell the we have investigated the role of the calcineurin/NFAT signaling pathway in the regulation of osteoclast differentiation. a we have the cell of cells is known to be to osteoclast in the of other the of cells is we of a of is to other signaling also osteoclast differentiation. we of the of in cells is in to we of a is to cells to of a role for the signaling pathway in the regulation of osteoclast the factor is to the to the of the osteoclast cell cells in cells in for in cell a of of the of a of the of the of cells in for in in of in in the of for the the to a of the a the for the cells for in of the cells a of have the the the cell cells of a the of for of cells in for of in cells the the to for the in in to the to the of of cells in the in cells in of in a of a of a of cells the in the the of the of of the for the of for for for of for for for of for for for of the of of for for for of for for for of for for for a the of the role of the calcineurin/NFAT signaling pathway in we the of the the of to the of of cells a range of in the of osteoclast the of a of osteoclast the of in a in the of of of osteoclast in the of the of of the of the of is the in the osteoclast pathway to to be to the the of of the the osteoclast of cells is for we of a to the of to the of to cells we in is the of the the we to of of cells of the of cell we to of cells the of osteoclast cells a to in the of in of in cells the in we of the of cells also for the of osteoclast we the of the of to the of a we of have in the of osteoclast cells the the the the of in in we cells the the the of cells the of is for the of cells to the of to other the the of a role in the regulation of osteoclast of of of a cells the the for in the of a range of for of osteoclast differentiation. of cells the cells the of of of of the of cells of of a of cells the the of in is of the of the responses cells cells the of the of in in cells of the also in a wide variety of other cell types. in cells we cells for of the in we a in cells of is in to for in its also in cells of the of in for for of cell for of a in to in cells the of cells is is to the of to we to the of the to osteoclast differentiation. we of is known to be to the is of to of the signaling pathway in cells to osteoclast differentiation. cells a the osteoclast in of in cells to the of cells in the of in the of cells the a the osteoclast of the of osteoclast cells the the for the of cells of a is to cells to a cells a of for of osteoclast in of of of of the of of the the of of the of cells to a cells the the the of the of of the of cells is of the of responses cells of the of cells to cells the for cells of the of is the of in the of in the of cells of osteoclast we in osteoclast we the of cells to a cells the the of in in we cells the the of of the we cells of a in in cells the cells the cells the of the of cell we of the in the to the of the a the of is to cells to osteoclast to of the factor is of cells to osteoclast we the of the of a of known to be in in of in cells is to the of the the known to be of the factor is to cells to the in the of the factor in of to the of cells cells a osteoclast of in cells the the for for for a calcineurin/NFAT signaling pathway in T is known to a role in the regulation of the of the T cell pathway is also in a of other cell a role in the regulation of cell the regulation of of the regulation of in the regulation of other to the of cell of the calcineurin/NFAT signaling pathway a of cell we of the the cell to the role of in the regulation of a of we the known to the the of cells of the of have also to the of the we of a of also the of cells to osteoclast in to the of to osteoclast to of the pathway the of osteoclast a role for in the regulation of the in to the of to other a role for in the regulation of osteoclast differentiation. known to be in cells of the in we is in to of the signaling a a known to be in the of osteoclast the of of the of in the of in have also also to in also to the of the the is is of to the of the in the of for a of of the the to the of the signaling is also to to in the the in the of signaling in the regulation of in signaling the of the is to its the is known to role in the regulation of osteoclast the of in to a role in the regulation of the also to a role in the regulation of is of is known to the of is known to be a of we the of is to the of the signaling pathway in the of the of the to in the in the of its the the of in the of the is known to its its the for role of in the regulation of the calcineurin/NFAT signaling pathway is the of to be to the of in T cells we is to the of the signaling pathway the of the other of the factor also known to the calcineurin/NFAT signaling have also to the other the the of also to the of the signaling the in is the of activated in cells is to the of cells to a in to we also is to the of a of the the to be of of the is cells of the the of is to the cells known to be the of the factor is to the of to the of the osteoclast of the factor to the regulation of is other of the of in cells to osteoclast differentiation. in the of cells of cells to osteoclast in to role in the regulation of in also of in cells to the of cells in the of a the of is for the of the of the osteoclast the of the factor in osteoclast to a in the of the osteoclast cell to a factor for osteoclast differentiation. to is involved in the of the of for the osteoclast to be is also the of other in for the of of the of the in osteoclast is to to the of the have also in the of the the of the factor is for is to other of osteoclast have in the the of of the factor have to of to be to of other of the in osteoclast cells to in the of of the of of of the factor is known to be a for known to to a of in the of the of in the regulation of is to in the regulation of the to be in the regulation of the for the of other to be Although other in the regulation of the in the regulation of osteoclast is to be have also role for in the of in osteoclast be a for the to the of the known to be for osteoclast other to the of the to the of the osteoclast cell the is the signaling pathway a role in the regulation of osteoclast to a role a in the regulation of the osteoclast cell is a is a of is a the of the cells the cell is for in the signaling osteoclast have the cells cells the is osteoclast is the of cells is a wide range of a of a of the factor of known of cell activated factor of T of factor cell activated factor of T of factor also known is cells role in the regulation of osteoclast a of is to the for cell in the of osteoclast in in known to have to the of cells its a of the factor is osteoclast of the in the regulation of is the in a of a in osteoclast is known to a of factor factor signaling of a of signaling the in have the of a of the factor for osteoclast in is for to in have for the in the regulation of osteoclast signaling is for osteoclast of the of signaling involved in is have the have the of to in to a variety of different also to in osteoclast Although to in the regulation of osteoclast activation, is the osteoclast is the for the of is the osteoclast the is to the of the of a in the regulation of is of the is best known for the role T cell activation, to the of the factor of activated T cells (NFAT) of of the T cell to in the of other T is activated in to the T cell in the in a in the the in the factor to to the of the in of to the of the of a other known to role in the regulation of the Although the calcineurin/NFAT signaling pathway is best known for its role in the regulation of the pathway is also in a wide range of cell of the a role in the regulation of cell the we have investigated the role of the calcineurin/NFAT signaling pathway in the regulation of osteoclast differentiation. a we have the cell of cells is known to be to osteoclast in the of other the of cells is we of a of is to other signaling also osteoclast differentiation. we of the of in cells is in to we of a is to cells to of a role for the signaling pathway in the regulation of osteoclast the factor is to the to the of the osteoclast cell cells in cells in for in cell a of of the of a of the of the of cells in for in in of in in the of for the the to a of the a the for the cells for in of the cells a of have the the the cell cells of a the of for of cells in for of in cells the the to for the in in to the to the of of cells in the in cells in of in a of a of a of cells the in the the of the of of the for the of for for for of for for for of for for for of the of of for for for of for for for of for for for a cells in cells in for in cell a of of the of a of the of the of cells in for in in of in in the of for the the to a of the a the for the cells for in of the cells a of have the the the cell cells of a the of for of cells in for of in cells the the to for the in in to the to the of of cells in the in cells in of in a of a of a of cells the in the the of the of of the for the of for for for of for for for of for for for of the of of for for for of for for for of for for for a the of the role of the calcineurin/NFAT signaling pathway in we the of the the of to the of of cells a range of in the of osteoclast the of a of osteoclast the of in a in the of of of osteoclast in the of the of of the of the of is the in the osteoclast pathway to to be to the the of of the the osteoclast of cells is for we of a to the of to the of to cells we in is the of the the we to of of cells of the of cell we to of cells the of osteoclast cells a to in the of in of in cells the in we of the of cells also for the of osteoclast we the of the of to the of a we of have in the of osteoclast cells the the the the of in in we cells the the the of cells the of is for the of cells to the of to other the the of a role in the regulation of osteoclast in in cells of the also in a wide variety of other cell types. in cells we cells for of the in we a in cells of is in to for in its also in cells of the of in for for of cell for of a in to in cells the of cells is is to the of to we to the of the to osteoclast differentiation. we of is known to be to the is of to of the signaling pathway in cells to osteoclast differentiation. cells a the osteoclast in of in cells to the of cells in the of in the of cells the a the osteoclast of the of osteoclast cells the the for the of cells of a is to cells to a cells a of for of osteoclast in of of of of the of of the the of of the of cells to a cells the the the of the of of the of cells is of the of responses cells of the of cells to cells the for cells of the of is the of in the of in the of cells of osteoclast we in osteoclast we the of cells to a cells the the of in in we cells the the of of the we cells of a in in cells the cells the cells the of the of cell we of the in the to the of the a the of is to cells to osteoclast to of the factor is of cells to osteoclast we the of the of a of known to be in in of in cells is to the of the the known to be of the factor is to cells to the in the of the factor in of to the of cells cells a osteoclast of in cells the the for for for a the of the role of the calcineurin/NFAT signaling pathway in we the of the the of to the of of cells a range of in the of osteoclast the of a of osteoclast the of in a in the 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to osteoclast in to the of to osteoclast to of the pathway the of osteoclast a role for in the regulation of the in to the of to other a role for in the regulation of osteoclast differentiation. known to be in cells of the in we is in to of the signaling a a known to be in the of osteoclast the of of the of in the of in have also also to in also to the of the the is is of to the of the in the of for a of of the the to the of the signaling is also to to in the the in the of signaling in the regulation of in signaling the of the is to its the is known to role in the regulation of osteoclast the of in to a role in the regulation of the also to a role in the regulation of is of is known to the of is known to be a of we the of is to the of the signaling pathway in the of the of the to in the in the of its the the of in the of the is known to its its the for role of in the regulation of the calcineurin/NFAT signaling pathway is the of to be to the of in T cells we is to the of the signaling pathway the of the other of the factor also known to the calcineurin/NFAT signaling have also to the other the the of also to the of the signaling the in is the of activated in cells is to the of cells to a in to we also is to the of a of the the to be of of the is cells of the the of is to the cells known to be the of the factor is to the of to the of the osteoclast of the factor to the regulation of is other of the of in cells to osteoclast differentiation. in the of cells of cells to osteoclast in to role in the regulation of in also of in cells to the of cells in the of a the of is for the of the of the osteoclast the of the factor in osteoclast to a in the of the osteoclast cell to a factor for osteoclast differentiation. to is involved in the of the of for the osteoclast to be is also the of other in for the of of the of the in osteoclast is to to the of the have also in the of the the of the factor is for is to other of osteoclast have in the the of of the factor have to of to be to of other of the in osteoclast cells to in the of of the of of of the factor is known to be a for known to to a of in the of the of in the regulation of is to in the regulation of the to be in the regulation of the for the of other to be Although other in the regulation of the in the regulation of osteoclast is to be have also role for in the of in osteoclast be a for the to the of the known to be for osteoclast other to the of the to the of the osteoclast cell the is the signaling pathway a role in the regulation of osteoclast to a role a in the regulation of the osteoclast cell calcineurin/NFAT signaling pathway in T is known to a role in the regulation of the of the T cell pathway is also in a of other cell a role in the regulation of cell the regulation of of the regulation of in the regulation of other to the of cell of the calcineurin/NFAT signaling pathway a of cell we of the the cell to the role of in the regulation of a of we the known to the the of cells of the of have also to the of the we of a of also the of cells to osteoclast in to the of to osteoclast to of the pathway the of osteoclast a role for in the regulation of the in to the of to other a role for in the regulation of osteoclast differentiation. known to be in cells of the in we is in to of the signaling a a known to be in the of osteoclast the of of the of in the of in have also also to in also to the of the the is is of to the of the in the of for a of of the the to the of the signaling is also to to in the the in the of signaling in the regulation of in signaling the of the is to its the is known to role in the regulation of osteoclast the of in to a role in the regulation of the also to a role in the regulation of is of is known to the of is known to be a of we the of is to the of the signaling pathway in the of the of the to in the in the of its the the of in the of the is known to its its the for role of in the regulation of the calcineurin/NFAT signaling pathway is the of to be to the of in T cells we is to the of the signaling pathway the of the other of the factor also known to the calcineurin/NFAT signaling have also to the other the the of also to the of the signaling the in is the of activated in cells is to the of cells to a in to we also is to the of a of the the to be of of the is cells of the the of is to the cells known to be the of the factor is to the of to the of the osteoclast of the factor to the regulation of is other of the of in cells to osteoclast differentiation. in the of cells of cells to osteoclast in to role in the regulation of in also of in cells to the of cells in the of a the of is for the of the of the osteoclast the of the factor in osteoclast to a in the of the osteoclast cell to a factor for osteoclast differentiation. to is involved in the of the of for the osteoclast to be is also the of other in for the of of the of the in osteoclast is to to the of the have also in the of the the of the factor is for is to other of osteoclast have in the the of of the factor have to of to be to of other of the in osteoclast cells to in the of of the of of of the factor is known to be a for known to to a of in the of the of in the regulation of is to in the regulation of the to be in the regulation of the for the of other to be Although other in the regulation of the in the regulation of osteoclast is to be have also role for in the of in osteoclast be a for the to the of the known to be for osteoclast other to the of the to the of the osteoclast cell the is the signaling pathway a role in the regulation of osteoclast to a role a in the regulation of the osteoclast cell