This paper is focused on the problem of determination of internal parameters of a fluidic lens composed of two immiscible liquids of different refractive index, which form a tunable refractive interface for changing the focal length of a lens. Formulas are derived for calculation of a radius of curvature of the internal interface between two liquids and refractive indices of liquids using the measurements of the focal length of the lens, positions of focal points, and transverse spherical aberration of the lens.