六烯酸
链脲佐菌素
化学
胰岛素
氧化应激
锌
胰岛素抵抗
糖尿病
脂肪酸
药理学
生物化学
内科学
内分泌学
多不饱和脂肪酸
医学
有机化学
作者
Jihan Hussein,Mohamed F. Attia,Mona A. El‐Bana,Sherien M. El‐Daly,Nadia A. Mohamed,Zakeria El-Khayat,Mehrez E. El‐Naggar
标识
DOI:10.1016/j.ijbiomac.2019.08.201
摘要
Our goal in this study is to improve the efficiency of docosahexaenoic acid (DHA) toward the enhancement of insulin signaling pathway in vivo via loading with zinc oxide nanoparticles (ZnO NPs). To this end, two consecutive steps were undertaken, preparation of ZnO NPs by one-step solid-state reaction in dry conditions and calcinated followed by loading DHA. Both developed nanoparticles, with and without DHA were then characterized by TEM, SEM, EDX, and Zetasizer. For comparison between free and loaded DHA, four groups of rats were prepared to receive different treatments. Group I; healthy rats (reference), group II; diabetes (streptozotocin-induced), group III and group IV are diabetes orally administered with free DHA and DHA-loaded ZnO NPs (10 mg/kg bw/day), respectively. Blood samples were collected and analyzed where the results demonstrated that fasting blood sugar and insulin resistance were significantly increased in diabetic group along with upgrading in oxidative stress parameters emphasizing the oxidative properties of streptozotocin. HPLC analysis of cell membrane fatty acids resulted in the reduction of omega-6 and 9 and elevation of omega-3 after free DHA and DHA-loaded ZnO NPs streptozotocin treatments. DHA-loaded ZnO NPs had high performance in enhancing insulin signaling pathway as expressed in changes of phosphatidylinositol 3-kinase (PI3K) levels.
科研通智能强力驱动
Strongly Powered by AbleSci AI