染色质
染色体构象捕获
染色体
计算生物学
进化生物学
生物
遗传学
DNA
基因
转录因子
增强子
作者
Nezih Karasu,Tom Sexton
出处
期刊:Methods in molecular biology
日期:2020-08-21
卷期号:: 19-34
被引量:4
标识
DOI:10.1007/978-1-0716-0664-3_3
摘要
Chromosome conformation capture and its variants have allowed chromatin topology to be interrogated at a superior resolution and throughput than by microscopic methods. Among the method derivatives, 4C-seq (circular chromosome conformation capture, coupled to high-throughput sequencing) is a versatile, cost-effective means of assessing all chromatin interactions with a specific genomic region of interest, making it particularly suitable for interrogating chromatin looping events. We present the principles and procedures for designing and implementing successful 4C-seq experiments.
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