The experiment was conducted to study the mensuration of IgG content in chickens′ mucosal immune system after Lasota vaccination. The changes of HI titer in immunized chickens′ tear, tracheal fluid and serum were measured in the experiment. The results showed that when mucosal immunity in NDV immunity is used, the IgG content of mucosal immune response positions is higher than that when humoral immunity is used; After the mucosal immunity, the stronger immune response is produced near the immunized position; HI titer in mucosal immune positions is higher than that in serum after the first immunity, but the result is reversed after the second immunity.