A study on the new extraction process of ursolic acid from the P.tomentosa (Thunb.)Steud leaves

The optimal process of extracting the effective component ursolic acid from P.tomentosa(Thunb.)Steud leaves is selected by using orthogonal test: the extraction is conducted twice,1 hour each time;the temperature is 85℃; the ethanol density is 90%～95%; the crude residue was suspended in 15～20 times PE (Bp 60～90℃) for 3～4 times (18～24 hours for each time),and then purified by 3 times EtOH for 2～3 times.The colorless ursolic acid needles was identified by organic spectral analysis such as IR,UV and MS.The content of ursolic acid is 96.9% by HPLC.