The Inhibitory Effect of the Serum Containing Liuyueqing on the Expression of HBsAg and HBeAg in the HepG2.2.15 Cells

Objective To observe the effect of the serum containing Liuyueqing(LYQ)on the expression of HBsAg and HBeAg in HepG2.2.15 cells.Methods The toxicity of the serum containing LYQ on HepG2.2.15 cells was detected by means of MTT.The inhibitory effects of the serum containing LYQ on the excreation of HBsAg and HBeAg in HePG 2.2.15 cells were evaluated with method of enzyme linked immunosorbent assay(ELISA).Results The dose which the serum containing LYQ could inhibit 50% HepG2.2.15 cells was 11.56 g.(kg.d)-1.The dose which the serum containing LYQ could inhibit 50% HBsAg was 0.178g.(kg.d)-1,and its treatment index(TI)on HBsAg was 64.94.The inhibitory effect of the serum containing LYQ on HBeAg was not obvious.Conclusion The serum containing LYQ can inhibit HBV significantly in vitro,and it is a low toxic drug.