Introduction: Garlic (Allium sativum) is widely used in cooking and in the treatment and prevention of various diseases. The literature lacks for toxicity and the effects that excessive consumption of garlic can cause the male reproductive system. The garlic oil comprises volatile compounds in their bulbs. This study aims to evaluate the morphometric of the seminiferous tubules with garlic oil supplemented mice. Methods: We treated 32 BALB/c mice divided into 4 experimental groups: 1,4 mg/kg (therapeutic dose); 14 mg/kg; 28 mg/kg; and control incorporated into feed meal. After the experimental period, the animals were sacrificed and had their testes