A signal on–off strategy based on the digestion of DNA cubes assisted by the CRISPR–Cas12a system for ultrasensitive HBV detection in solid-state nanopores

A sensing strategy for the ultrasensitive detection of HBV in nanopores was constructed by digestion of elements of DNA cubes and the use of CRISPR–Cas12a technology. Coupling with PCR preamplification technology enabled HBV-positive or -negative classification.