High molecular weight laminarin/AgNPs-impregnated PVA based in situ hydrogels accelerated diabetic wound healing

Diabetes exhibits several long-term serious health complications, including healing-impaired wounds, which result in substantial clinical challenges, warranting the need to develop efficient wound dressings. Herein, multifunctional in situ hydrogels as diabetic wound dressings were accomplished by concomitant spraying of 3-aminophenyl boronic acid (PBA)- grafted dialdehyde laminarin (LamPBA) and silver nanoparticles-impregnated polyvinyl alcohol (PVA-AgNPs). The hydrogels with and without AgNPs (F2 and F1, respectively) conferred excellent rheological characteristics and acceptable antibacterial activity (up to 80 % reduction in survival) against E. coli and S. aureus. Cellular experiments revealed that the hydrogels obviously promoted the proliferation and migration of HaCaT and NIH/3 T3 cells. In diabetic mice, the hydrogels accomplished ~90 % wound closure by day 9, outperforming controls (65-70 %). Additionally, F1 and F2 considerably increased the CD206/CD86 ratio (46 ± 5 and 62 ± 7, respectively) compared to diabetic (0.6 ± 0.1) and nondiabetic (2 ± 0.2) controls. Moreover, the hydrogels demonstrated remarkably triggered epidermal tissue regeneration, collagen deposition, antioxidant defense and angiogenesis. The hydrogels containing AgNPs portrayed superior wound healing potential over AgNP-free hydrogels. Overall, the in situ hydrogels (LamPBA/PVA-AgNPs, F2) could augment the diabetic wound healing rates in an effective manner and be utilized as promising wound care biomaterials.