氧化应激
药理学
小岛
胰腺
体内
细胞凋亡
细胞毒性
半胱氨酸蛋白酶3
细胞内
抗氧化剂
谷胱甘肽
化学
胰岛素
体外
程序性细胞死亡
生物
生物化学
内分泌学
酶
生物技术
作者
Jiayuan Luo,Zefu Chen,Qingbin Guo,Yangyang Chai,Yihong Bao
标识
DOI:10.1016/j.fct.2023.113724
摘要
The damage of islet cells caused by oxidative stress is closely related to diabetes. The aim of this study is to investigate the protective effect of saponins isolated from polygonatum sibiricum (PSS) on pancreas injury by using in vitro and in vivo models. The oxidative stress model of RIN-m5F cells induced by H2O2 was established. We found that PSS could decrease the apoptosis of RIN-m5F cells under oxidative stress. After PSS treatment, ROS and MDA levels in cells significantly decreased. Moreover, the levels of SOD and GSH were significantly increased. PSS could increase the insulin secretion level of cells under oxidative stress. The expression level of intracellular Bcl-2 increased, and the expression levels of Bax, caspase-3, caspase-8, and caspase-9 decreased significantly. In addition, the type 2 diabetes mouse model was established. The results showed that PSS had a protective effect on the injury of the pancreas in T2DM mice. PSS can relieve oxidative stress and high glucose-mediated pancreas cytotoxicity. PSS may be a promising candidate for diabetes intervention and functional foods.
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