One-step generation of core–shell biomimetic microspheres encapsulating double-layer cells using microfluidics for hair regeneration

微球 微流控 材料科学 壳体(结构) 图层(电子) 生物医学工程 再生(生物学) 芯(光纤) 纳米技术 仿生材料 化学工程 复合材料 细胞生物学 工程类 生物
作者
Junfei Huang,Danlan Fu,Xiaoqi Wu,Yue Li,Bowen Zheng,Zhen Liu,Yi Zhou,Yuyang Gan,Yong Miao,Zhiqi Hu
出处
期刊:Biofabrication [IOP Publishing]
卷期号:15 (2): 025007-025007 被引量:20
标识
DOI:10.1088/1758-5090/acb107
摘要

Abstract Tissue engineering of hair follicles (HFs) has enormous potential in the treatment of hair loss. HF morphogenesis is triggered by reciprocal interactions between HF germ epithelial and mesenchymal layers. Here, a microfluidic-assisted technology is developed for the preparation of double aqueous microdroplets that entrap double-layer cells and growth factors to ultimately be used for hair regeneration. Mouse mesenchymal cells (MSCs) and epidermal cells (EPCs) are encapsulated in gelatin methacrylate (GelMA) cores and photo-curable catechol-grafted hyaluronic acid (HAD) shells to fabricate GelMA-MSC/HAD-EPC (G/HAD) microspheres. The findings show that the G/HAD microspheres exhibit ultrafast gelation, aqueous phase separation, superior biocompatibility, and favorable wet adhesion properties. G/HAD microspheres can also support cell proliferation and sustain growth factor release. These composite cell microspheres are capable of efficient HF generation upon transplantation into the dorsal dermis of nude mice. This finding facilitates the large-scale preparation of approximately 80 double-layer cell spheres per min. This simple double-layer cell sphere preparation approach is a promising strategy for improving current hair-regenerative medicine techniques and can potentially be applied along with other organoid techniques for extended applications.
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