胰腺上皮内瘤变
胰腺癌
病理
上皮内瘤变
医学
病态的
胰腺
多光子荧光显微镜
病变
荧光显微镜
内科学
癌症
荧光
光学
胰腺导管腺癌
前列腺
物理
作者
Miao Jiang,Zheng Zhang,Zeng Xiong,Xingxin Huang,Shichao Zhang,Zhenlin Zhan,Jianxin Chen,Linying Chen,Lianhuang Li
标识
DOI:10.1002/jbio.202300417
摘要
Pancreatic intraepithelial neoplasia (PanIN) is the most common precursor lesion that has the potential to progress to invasive pancreatic cancer, and early and rapid detection may offer patients a chance for treatment before the development of invasive carcinoma. Therefore, the identification of PanIN holds significant clinical importance. In this study, we first used multiphoton microscopy (MPM) combining two-photon excitation fluorescence and second-harmonic generation imaging to label-free detect PanIN and attempted to differentiate between normal pancreatic ducts and different grades of PanIN. Then, we also developed an automatic image processing strategy to extract eight morphological features of collagen fibers from MPM images to quantify the changes in collagen fibers surrounding the ducts. Experimental results demonstrate that the combination of MPM and quantitative information can accurately identify normal pancreatic ducts and different grades of PanIN. This study may contribute to the rapid diagnosis of pancreatic diseases and may lay the foundation for further clinical application of MPM.
科研通智能强力驱动
Strongly Powered by AbleSci AI