生物
病菌
陈皮
转录组
柑橘类水果
芸香科
柑橘×冬青
唾液腺
微生物学
病毒学
植物
园艺
橙色(颜色)
遗传学
基因
半翅目
基因表达
生物化学
作者
Shu-Zhen Zhao,Xueqin Ran,Yan Huang,Wen Sang,Bugenimana Eric Derrick,Bao‐Li Qiu
出处
期刊:Bulletin of Entomological Research
[Cambridge University Press]
日期:2024-03-06
卷期号:: 1-20
标识
DOI:10.1017/s0007485324000038
摘要
Abstract The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is the key vector insect transmitting the Candidatus Liberibacter asiaticus ( C Las) bacterium that causes the devastating citrus greening disease (Huanglongbing, HLB) worldwide. The D. citri salivary glands (SG) exhibit an important barrier against the transmission of HLB pathogen. However, knowledge on the molecular mechanism of SG defence against C Las infection is still limited. In the present study, we compared the SG transcriptomic response of C Las-free and C Las-infected D. citri using an illumine paired-end RNA sequencing. In total of 861 differentially expressed genes (DEGs) in the SG upon C Las infection, including 202 upregulated DEGs and 659 downregulated DEGs were identified. Functional annotation analysis showed that most of the DEGs were associated with cellular processes, metabolic processes, and the immune response. Gene ontology and Kyoto Encyclopaedia of Genes and Genomes enrichment analyses revealed that these DEGs were enriched in pathways involving carbohydrate metabolism, amino acid metabolism, the immune system, the digestive system, the lysosome, and endocytosis. A total of 16 DEGs were randomly selected to further validate the accuracy of RNA-Seq dataset by reverse-transcription quantitative polymerase chain reaction. This study provides substantial transcriptomic information regarding the SG of D. citri in response to C Las infection, which may shed light on the molecular interaction between D. citri and C Las, and provides new ideas for the prevention and control of citrus psyllid.
科研通智能强力驱动
Strongly Powered by AbleSci AI