泊洛沙姆
胶束
Zeta电位
细胞毒性
基因传递
转染
化学
细胞内
动态光散射
泊洛沙姆407
临界胶束浓度
生物物理学
粒径
芘
核化学
体外
色谱法
材料科学
纳米技术
生物化学
有机化学
共聚物
纳米颗粒
聚合物
基因
生物
水溶液
物理化学
作者
Hebin Wang,Leixiang Yang,Xingang Liu,Jun Zhou,Qingqing Wang,Guping Tang
出处
期刊:PubMed
日期:2017-03-25
卷期号:46 (2): 134-143
摘要
Objective: To prepare and characterize Pluronic-PEI micelles as a drug/gene delivery system. Methods: We used the low-molecular-weight PEI as a cross-linking agent to prepare the Pluronic-PEI micelles. The particle size, zeta potential and critical micelle concentration (CMC) were measured by dynamic light scattering (DLS) and pyrene fluorescence probe. The cytotoxicity, transfection efficiency and the impact on the intracellular ATP and P-gp levels of Pluronic-PEI micelles were investigated at the cellular level. Results: Pluronic-PEI micelles were successfully prepared with a suitable particle size (120-180 nm), zeta potential (+6-+9 mv), and a good ability to carry the drug/gene. An in-vitro study showed that Pluronic-PEI had low cytotoxicity, and the P123-PEI600 possessed high gene transfection efficiency and could downregulate the intracellular ATP and P-gp levels. Conclusion: Pluronic-PEI is a good drug/gene delivery system, and P123-PEI600 is an ideal vector, which may be used in the combination therapy for reversing multidrug resistance.
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