肌发生
生物
黑腹果蝇
计算生物学
模式生物
心肌细胞
基因
遗传学
细胞生物学
作者
Anton L. Bryantsev,Lizzet Castillo,Sandy T. Oas,Maria B. Chechenova,Tracy E. Dohn,TyAnna L. Lovato
出处
期刊:Methods in molecular biology
日期:2019-01-01
被引量:3
标识
DOI:10.1007/978-1-4939-8897-6_16
摘要
Drosophila is a useful model organism for studying the molecular signatures that define specific muscle types during myogenesis. It possesses significant genetic conservation with humans for muscle disease causing genes and a lack of redundancy that simplifies functional analysis. Traditional molecular methods can be utilized to understand muscle developmental processes such as Western blots, in situ hybridizations, RT-PCR and RNAseq, to name a few. However, one challenge for these molecular methods is the ability to dissect different muscle types. In this protocol we describe some useful techniques for extracting muscles from the pupal and adult stages of development using flight and jump muscles as an example.
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