氧化磷酸化
糖酵解
三磷酸腺苷
细胞外
细胞内
线粒体
细胞生物学
海马
生物化学
癌变
生物
化学
新陈代谢
基因
动物
出处
期刊:Methods in molecular biology
日期:2019-01-01
卷期号:: 353-363
被引量:80
标识
DOI:10.1007/978-1-4939-9027-6_18
摘要
A large amount of energy used for nutrient processing and cellular functions is essential for tumorigenesis. Total intracellular adenosine triphosphate (ATP) is mainly generated by glycolysis and mitochondrial oxidative phosphorylation. Here, we provide a protocol for measurements of energy metabolism in cancer cells by using Seahorse XF24 Extracellular Flux analyzer. Specifically, this machine measures glycolysis by analyzing the extracellular acidification rate (ECAR) and measures mitochondrial oxidative phosphorylation on the basis of the oxygen consumption rate (OCR), through real-time and live cell analysis. This protocol is provided for researchers who are unfamiliar with the method and to aid them in carrying out the technique successfully.
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