The establishment of zebrafish Dio3b gene knockout model

Objective To set up the model of deiodinase (Dio) 3b-/- zebrafish and to observe the effect of which on embryo development. Methods The zebrafish model of Dio3b-/- was set up by CRISPR-Cas9 gene editing technology, PCR and sequencing was used to confirm the efficiency of deletion. The heart rate of embryos at 48 hours post fertilization was counted. The locomotor activity of 5-7 days post fertilization larve was detected using behavior tracking system. Results The model of Dio3b knockout zebrafish was set up successfully. The heart rate of embryos Dio3b-/- increased (P<0.001) and the locomotor activity of 5-7 days post fertilization larves lacking Dio3b gene increased (P<0.05) significantly compared with that of wild type control respectively. Conclusion The deletion of zebrafish Dio3b gene results in the phenotype of hyperthyroidism and the model of Dio3b-/- is proper for studying the effect of partial excess thyroid hormone on embryo development. (Chin J Endocrinol Metab, 2018, 34: 790-794) Key words: Zebrafish; CRISPR-Cas Systems; Iodothyronine deiodinase type 3; Thyroid hormones; Heart rate