生物传感器
发光
检出限
银纳米粒子
纳米颗粒
材料科学
色谱法
免疫分析
化学
纳米技术
光电子学
医学
免疫学
抗体
作者
Weipan Peng,Yi Qin,Wenna Li,Minghui Chen,Dianming Zhou,Hengxuan Li,Jingyu Cui,Jin Chang,Sheng Xie,Xiaoqun Gong,Ben Zhong Tang
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2020-06-24
卷期号:5 (7): 1912-1920
被引量:36
标识
DOI:10.1021/acssensors.9b02355
摘要
Sensitive and portable quantification of biomarkers has particular significance in the monitoring and treatment of clinical diseases. Conventional immunoassays were accustomed to introducing or incorporating enzymes for signal amplification, which commonly suffered from poor stability and inferior tolerance. Herein, we constructed a novel nonenzyme amplification methodology based on fluorogenic Ag+-tetrazolate aggregation coupled with silver corrosion sensitization for biomarker determination. A significant cascade enhancement strategy was achieved by the valid aggregation luminescence caused by the potent disintegration of silver nanoparticles. Furthermore, efficient magnetic separation was also combined and performed for the rapidity and simplicity of operation. As the target, the detection limit of prostate-specific antigen was 15.66 pg/mL in our designed biosensor. Besides, a good linear relationship was obtained. The designed biosensor demonstrated good specificity and was successfully applied to clinical serum sample detection.
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