Amelioration of LPS‐induced inflammatory response and oxidative stress by astaxanthin in Channa argus lymphocyte via activating glucocorticoid receptor signalling pathways

Aquaculture ResearchVolume 51, Issue 7 p. 2687-2697 ORIGINAL ARTICLE Amelioration of LPS-induced inflammatory response and oxidative stress by astaxanthin in Channa argus lymphocyte via activating glucocorticoid receptor signalling pathways Mu-Yang Li, Mu-Yang Li College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, Jilin, ChinaSearch for more papers by this authorChun-Shan Gao, Chun-Shan Gao Freshwater Fisheries Research Institute of Jilin Province, Changchun, Jilin, ChinaSearch for more papers by this authorXiao-Yan Du, Xiao-Yan Du Freshwater Fisheries Research Institute of Jilin Province, Changchun, Jilin, ChinaSearch for more papers by this authorLei Zhao, Lei Zhao College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, Jilin, ChinaSearch for more papers by this authorXiao-Tian Niu, Xiao-Tian Niu College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, Jilin, ChinaSearch for more papers by this authorGui-Qin Wang, Corresponding Author Gui-Qin Wang [email protected] [email protected] orcid.org/0000-0001-6233-7694 College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, Jilin, ChinaFirst corresponding author. Correspondence Gui-Qin Wang and Dong-Ming Zhang, College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin 130118, China. Emails: [email protected]; [email protected]Search for more papers by this authorDong-Ming Zhang, Corresponding Author Dong-Ming Zhang [email protected] [email protected] College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, Jilin, China Correspondence Gui-Qin Wang and Dong-Ming Zhang, College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin 130118, China. Emails: [email protected]; [email protected]Search for more papers by this author Mu-Yang Li, Mu-Yang Li College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, Jilin, ChinaSearch for more papers by this authorChun-Shan Gao, Chun-Shan Gao Freshwater Fisheries Research Institute of Jilin Province, Changchun, Jilin, ChinaSearch for more papers by this authorXiao-Yan Du, Xiao-Yan Du Freshwater Fisheries Research Institute of Jilin Province, Changchun, Jilin, ChinaSearch for more papers by this authorLei Zhao, Lei Zhao College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, Jilin, ChinaSearch for more papers by this authorXiao-Tian Niu, Xiao-Tian Niu College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, Jilin, ChinaSearch for more papers by this authorGui-Qin Wang, Corresponding Author Gui-Qin Wang [email protected] [email protected] orcid.org/0000-0001-6233-7694 College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, Jilin, ChinaFirst corresponding author. Correspondence Gui-Qin Wang and Dong-Ming Zhang, College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin 130118, China. Emails: [email protected]; [email protected]Search for more papers by this authorDong-Ming Zhang, Corresponding Author Dong-Ming Zhang [email protected] [email protected] College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, Jilin, China Correspondence Gui-Qin Wang and Dong-Ming Zhang, College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin 130118, China. Emails: [email protected]; [email protected]Search for more papers by this author First published: 24 March 2020 https://doi.org/10.1111/are.14608Citations: 3 Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract The present study was conducted to evaluate the effects of astaxanthin (AST) against lipopolysaccharide (LPS)-induced lymphocyte viability, ultrastructural lesions, apoptosis, oxidative stress and inflammatory responses in Channa argus. Lymphocytes exposed to more than 10 μg/ml LPS alone for 24 hr showed significantly decreased cell viability, elevated nitric oxide (NO) and malondialdehyde (MDA), lactate dehydrogenase (LDH) contents, and increased nuclear factor κB p65 (NF-κB p65), myeloid differential protein-88 (MyD88), tumour necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-8 (IL-8), caspase-3, caspase-8 and caspase-9 gene expression. LPS at a concentration of 10 μg/ml could induce oxidative stress and inflammatory responses in lymphocytes. The activities of antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD)) were significantly decreased after exposure to 10 μg/ml LPS. Besides, AST strikingly antagonized the LPS-induced negative effects. AST significantly increased the expression of HSP70, HSP90, IκB-α, and glucocorticoid receptor (GR) and decreased inflammatory responses. Further study showed that AST can activate GR signalling pathway and inhibit p65 phosphorylation. In addition, AST attenuated LPS-induced apoptosis, mitochondrial swelling, degeneration and vacuolization. Collectively, these findings suggest that AST has protective roles in LPS-induced cell damage via modulating GR activation in C. argus lymphocytes. CONFLICT OF INTEREST No conflict of interest exits in the submission of this manuscript, and manuscript is approved by all authors for publication. Open Research DATA AVAILABILITY STATEMENT Research data are not shared. Citing Literature Volume51, Issue7July 2020Pages 2687-2697 RelatedInformation