增殖细胞核抗原
细胞凋亡
西罗莫司
流式细胞术
男科
增生
医学
免疫组织化学
细胞生长
生物
病理
外科
免疫学
生物化学
遗传学
作者
Xue Tai,Ying Shen,Huajie Zhao,Zhaoge Wang,Wenjie Guan,Xin Kang,Wenqi Guo
出处
期刊:PubMed
日期:2020-09-30
卷期号:40 (9): 1346-1352
标识
DOI:10.12122/j.issn.1673-4254.2020.09.19
摘要
To study the effect of rapamycin on scar formation in rabbit eyes following filtering operation and explore the possible mechanism.Ninety-six healthy adult rabbits were subjected to trabeculectomy of the left eye and subsequently randomly divided into 4 groups (n=24) for treatment with castor oil (control) or rapamycin (1%, 3%, or 5%) eye drops of the operated eyes 4 times a day. The morphology and function of the filtering blebs of the rabbits were compared at 7, 14, 21 and 28 days after the operation; at each of the time points, 6 rabbits from each group were euthanized for detection of expressions of proliferating cell nuclear antigen (PCNA) and α-smooth muscle actin (α-SMA) in the tissues in the surgical area using immunohistochemistry. Cultured rabbit subconjunctival fibroblasts (RTFSs) were treated with different concentrations of rapamycin (0.06, 0.25, 1, and 4 mg/L) and the cell apoptosis was detected using flow cytometry.In the first, second and third weeks after the operation, the rate of functional follicle formation was significantly higher in the 3 rapamycin groups than in the control group (P < 0.05), and the number of α- SMA-positive fibroblasts decreased over time in the 3 rapamycin groups. In cultured RTFSs, treatment with rapamycin at different concentrations resulted in increased apoptosis of the cells, and rapamycin above 0.25 mg/L significantly increased the cell apoptosis in a dose-dependent manner.Rapamycin can inhibit hyperplasia of the filtering passage tissue, helps to preserve the functional filtering blebs and prolong their life span, and induces apoptosis of RTFS.
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