小圆圈
dna疫苗
免疫系统
生物
Cre重组酶
质粒
抗原
体内
转染
重组酶
病毒学
免疫学
DNA
遗传学
转基因
基因
免疫
重组
转基因小鼠
作者
Yanlong Jiang,Genhua Yang,Chunfeng Wang
出处
期刊:Methods in molecular biology
日期:2020-08-22
标识
DOI:10.1007/978-1-0716-0872-2_1
摘要
Minicircle DNA (mcDNA) has been considered to be an alternative choice of traditional DNA vaccine due to its much smaller size, resulting in more efficient antigen synthesis, enhanced and long-lasting adaptive immune response, especially cellular immune response. However, the disadvantages such as relative high cost and labor intensiveness severely restrict its direct application in the field of veterinary vaccine. Here, we describe a novel Cre Recombinase-mediated In vivo McDNA platform, named CRIM, in which the parental plasmid could spontaneously transform into mcDNA by itself after transfection or oral administration. This CRIM vaccine platform might serve as a novel oral antigen delivery system for any infectious diseases, especially for veterinary application.
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