生物
生长素
习惯
多年生植物
转录组
植物
饲料
基因
基因表达
遗传学
心理学
心理治疗师
作者
Lin Ma,Dengxia Yi,Wenlong Gong,Pan Gong,Zan Wang
摘要
Abstract Astragalus adsurgens Pall., a perennial legume native to China, is commonly used as a forage crop. And it has great value for sustainable development of grasslands in arid and semi arid regions. However, to date, little is known regarding the A. adsurgens genome, and no studies have determined whether it would be possible to improve the germplasm of A. adsurgens through genetic modification. In this study, we used an RNA‐seq protocol to generate a de novo transcriptome including 151,516 unigenes of A. adsurgens . We compared the transcriptomes of A. adsurgens having different growth habits (prostrate/erect) and identified 14,133 single nucleotide polymorphism sites (SNP) in 8,139 unigenes. Differential expression gene (DEG) analysis suggested that 10,982 unigenes were up‐regulated in the prostrate plant relative to the erect plant, while 10,607 unigenes were down‐regulated. Of the 21,589 DEG, Unigene72782_All ( LAX4 ) and CL12494.Contig3_All ( TIR1 ), an auxin transporter gene and an auxin transport inhibitor gene, respectively, were predicted to influence the growth habit of A. adsurgens , which were verified by qRT‐PCR in these phenotypes. These results suggest that auxin transport was more active in the prostrate plant than in the erect plant, resulting in asymmetric distribution of auxin that affects the growth habit of A. adsurgens . Overall, this study may provide a basis for future research on key genes in A. adsurgens and may deepen our understanding of the molecular mechanisms regulating plant growth habit.
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