小檗碱
黄连
黄连碱
根茎
生物
毛茛科
转录组
木质部
黄连
植物
WRKY蛋白质结构域
生物碱
巴马汀
基因
基因表达
生物化学
病理
中医药
替代医学
医学
作者
Xiaomeng Liu,Junping Tan,Shuiyuan Cheng,Zexiong Chen,Jiabao Ye,Jiarui Zheng,Feng Xu,Weiwei Zhang,Yongling Liao,Xiaoyan Yang
标识
DOI:10.1016/j.scienta.2021.110585
摘要
Berberine is the primary medicinal component of Coptis chinensis, a perennial herb in Ranunculaceae. It has pharmacological effects, such as clearing away heat and dampness, purging fire and detoxifying, and resisting oxidation, and is widely used in clinical treatment. Anatomical analysis of the organs showed that berberine is mainly deposited in the xylem of the adventitious roots and rhizomes, as well as in thick-walled tissue layers containing vascular bundles in the leaves and petioles of C. chinensis. The content of total alkaloids in C. chinensis was highest in the rhizomes, among which berberine (54.44%) and coptisine (20.62%) were the main alkaloids. Furthermore, comparative transcriptome analysis of the leaves, petioles, rhizomes, and adventitious roots of C. chinensis was used to reveal the molecular regulation of alkaloid biosynthesis. A total 38 differentially expressed structural genes (DEGs) and 22 differentially expressed transcription factors were related to berberine biosynthesis in C. chinensis. Among these DEGs, the expression level of 13 genes was positively correlated with berberine content, including candidate structural genes (TYR, TYDC, TH, and PPO) and transcription factors (bHLH and WRKY), which may play key roles in berberine accumulation in C. chinensis. The results provide resources and technical support for improving the content of berberine in C. chinensis by genetic engineering, thus providing a theoretical foundation for enhancing the medicinal value of C. chinensis.
科研通智能强力驱动
Strongly Powered by AbleSci AI