DNA
纳米技术
光子上转换
材料科学
适体
信号(编程语言)
化学
光电子学
计算机科学
发光
生物
分子生物学
生物化学
程序设计语言
作者
Chi Yao,Jianpu Tang,Chenxu Zhu,Sen Yang,Han Tang,Luxi Dong,Chengqi Zhang,Qianyun Tang,Peifeng Liu,Dayong Yang
出处
期刊:Nano Today
[Elsevier]
日期:2022-02-01
卷期号:42: 101352-101352
被引量:19
标识
DOI:10.1016/j.nantod.2021.101352
摘要
The real-time drug-release monitoring is important for pharmacokinetic study toward clinical applications. The accuracy is usually severely affected by the fluctuation of complex biological microenvironment and dosage variation. Herein, a novel signal processor, upconversion nanoparticle (UCNP)-DNA nanocomplex (UCDC) was constructed via rational DNA design, enabling NIR-excited real-time in vivo drug-release monitoring, and realizing real-time pharmacokinetic monitoring. The signal processor was constructed by interfacial assembly of circular DNA on UCNP and the subsequent epitaxial assembly of ultralong DNA. Ultralong DNA chain was programed with multiple desired functional regions: double-stranded region for chemotherapy drugs integrating, DNA aptamer for tumor cells targeting and hairpin/i-motif switchable structure for tumor microenvironment responsive drug release. Based on filter effect of loaded drug molecules, efficient switch of real-time drug-release to optical signal was realized. For signal output, the ratiometer of red upconversion emission (UCLR) and NIR upconversion emission (UCLNIR) was monitored (UCLR/UCLNIR), with UCLNIR as an internal reference. In a breast cancer mouse model, the signal processor system achieved accurate and reliable localized drug-release monitoring, which effectively minimized the influence of the internal microenvironment and dosage variation.
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