双性恋
生物
性腺
女性化(社会学)
遗传学
基因
性腺发育
内分泌学
核糖核酸
社会科学
RNA剪接
社会学
作者
Hong Jo Lee,Minseok Seo,Hee Jung Choi,Deivendran Rengaraj,Kyung Min Jung,Jin Se Park,Kyung Youn Lee,Young Min Kim,Kyung Je Park,Soo Taek Han,Kyu Hyuk Lee,Haibo Yao,Jae Yong Han
标识
DOI:10.1096/fj.202100902r
摘要
Compared with the well-described XY sex determination system in mammals, the avian ZW sex determination system is poorly understood. Knockdown and overexpression studies identified doublesex and mab-3-related transcription factor 1 (DMRT1) as the testis-determining gene in chicken. However, the detailed effects of DMRT1 gene disruption from embryonic to adult development are not clear. Herein, we have generated DMRT1-disrupted chickens using the clustered regularly interspaced short palindromic repeats-associated protein 9 system, followed by an analysis of physiological, hormonal, and molecular changes in the genome-modified chickens. In the early stages of male chicken development, disruption of DMRT1 induced gonad feminization with extensive physiological and molecular changes; however, functional feminine reproductivity could not be implemented with disturbed hormone synthesis. Subsequent RNA-sequencing analysis of the DMRT1-disrupted chicken gonads revealed gene networks, including several novel genes linearly and non-linearly associated with DMRT1, which are involved in gonad feminization. By comparing the gonads of wild type with the genome-modified chickens, a set of genes were identified that is involved in the ZW sex determination system independent of DMRT1. Our results extend beyond the Z-dosage hypothesis to provide further information about the avian ZW sex determination system and epigenetic effects of gonad feminization.
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