生物
CD3型
内化
主要组织相容性复合体
细胞质
T细胞受体
分子生物学
流式细胞术
内吞作用
抗原
细胞生物学
T细胞
细胞培养
作者
Yasuhiro Yamane,Maritza I. Perez,Richard L. Edelson,Nicodemo Agostino,Benvenuto Pernis
标识
DOI:10.1016/0008-8749(91)90370-q
摘要
We investigated the expression of the T cell receptor (TCR)/CD3 complex on a CD4-positive human T cell lymphoma cell line treated with phorbol myristate acetate (PMA) and/or CA2+ ionophore using fluorescence flow cytometry and fluorescence microscopic analysis. PMA induced a significant decrease in the expression of the CD3 complex on the cell membranes. Fluorescence microscopy confirmed that the down regulation is due to internalization of the antigens. Ca2+ ionophore treatment had no effect on the internalization of the CD3 complex. Double staining revealed that the vesicles containing the internalized CD3 complex and those containing intra-cytoplasmic class I major histocompatibility complex antigen had similar distribution in the PMA-stimulated cells, implying coexistence of these two antigens in a cytoplasmic perinuclear distribution.
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