尿素酶
尿素
双乙酰
化学
色谱法
水解
氨基脲
生物化学
有机化学
作者
David F. Lambert,John E. Sherwood,Paul S. Francis
摘要
Although the dominant methods for the determination of urea in clinical applications incorporate selective enzymatic hydrolysis of urea, the determination of urea in soil extracts is complicated by the presence of urease inhibitors. The spectrophotometric determination of urea with an acidic solution diacetyl monoxime and semicarbazide is a viable option but traditional manual procedures are time-consuming. New variations on these procedures, based on microplates or flow-injection analysis methodologies, allow a far greater number of samples to be analysed with high precision and sensitivity.
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