枯草芽孢杆菌
同源重组
细菌人工染色体
生物
遗传学
基因组
DNA
基因组DNA
穿梭机载体
基因组文库
重组
遗传重组
同源染色体
大肠杆菌
重组DNA
计算生物学
基因
载体(分子生物学)
基序列
细菌
作者
Shinya Kaneko,Takashi Takeuchi,Mitsuhiro Itaya
标识
DOI:10.1016/j.jbiotec.2008.12.007
摘要
A Bacillus subtilis genome (BGM) vector system using homologous recombination was applied to connect two contiguous BAC clones covering the entire 355-kb transcription unit of the mouse jumonji genomic region. Results from the convenient genomic manipulation indicated that the BGM system facilitates the connection of DNAs from a BAC library without exchange and deletion of original sequence, which can expand large-sized DNA construction beyond BAC-building in Escherichia coli.
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