核酸酶
DNA
核糖核酸
核酸酶保护试验
分子生物学
化学
凝胶电泳
计算生物学
生物
生物化学
基因
非编码RNA
作者
Michael R. Green,Joseph Sambrook
出处
期刊:CSH Protocols
[Cold Spring Harbor Laboratory Press]
日期:2021-05-01
卷期号:2021 (5): pdb.prot101824-pdb.prot101824
被引量:7
标识
DOI:10.1101/pdb.prot101824
摘要
This protocol provides details for nuclease S1 mapping of mRNA using a uniformly labeled, single-stranded DNA probe. DNA-RNA hybrids are generated, which are subsequently digested with nuclease S1. The digestion products are separated using gel electrophoresis and analyzed by radiography.
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