An axial superresolution diffraction theory is developed in a two-photon microfabrication system. This method can improve the axial superresolution of the two-photon microfabrication system. A theoretical analysis of the photosensitive resin is discussed based on the exciting power and the concentration of free radical theory. Simulated results of the two-photon microfabrication verify the method and show that it can provide insight into the microfabrication system.