效力
柠檬酸钠
血凝素(流感)
放射免疫扩散
化学
免疫原性
重组DNA
二硫键
色谱法
微生物学
生物化学
生物
抗原
免疫学
抗体
体外
医学
基因
病理
作者
David G. Rhodes,Kathy Holtz,Pam Robinson,Keyang Wang,Clifton E. McPherson,Manon Cox,Indresh K. Srivastava
出处
期刊:Vaccine
[Elsevier]
日期:2015-11-01
卷期号:33 (44): 6011-6016
被引量:9
标识
DOI:10.1016/j.vaccine.2015.09.029
摘要
This study was designed to improve the stability of liquid formulations of recombinant influenza hemagglutinin (rHA) and to understand the mechanism of early loss of potency for rHA. The potency of rHA derived from several influenza strains was determined using single radial immunodiffusion (SRID), and the structure of the rHA was characterized using SDS-PAGE and dynamic light scattering. rHA formed disulfide cross-linked multimers, and potency decreased during extended storage. To reduce disulfide-mediated cross-linking and early potency loss, rHA was formulated with sodium thioglycolate (STG) and citrate. Addition of 80 mM STG and 55 mM sodium citrate inhibited disulfide-mediated cross-linking without affecting protein function for each rHA tested. The shelf life of the rHA formulation with STG-citrate, based on potency as determined by SRID, was extended as much as 20-fold, compared to a control formulation without STG-citrate. STG-citrate did not have a significant effect on the immunogenicity of H1 A/California/7/2009 rHA in mice.
科研通智能强力驱动
Strongly Powered by AbleSci AI