牙周炎
再生(生物学)
化学
炎症
牙槽
体内
Wnt信号通路
间充质干细胞
活性氧
二硫苏糖醇
间质细胞
细胞生物学
骨吸收
牙骨质
牙周纤维
癌症研究
免疫学
生物化学
牙科
信号转导
医学
生物
内科学
生物技术
酶
牙本质
作者
Shiyue Liu,Yanan Wang,Lu Yu,Jianhua Li,Shaohua Ge
标识
DOI:10.1016/j.cej.2021.134308
摘要
Periodontitis is characterized by local inflammatory response and alveolar bone resorption induced by pathogenic microorganisms colonization. The increase in reactive oxygen species (ROS) levels not only aggravates the local inflammation, but also inhibits the process of osteogenesis. The coupled process of reducing ROS level and reshaping the activity of osteoblasts is prerequisite to facilitate in situ periodontal tissue regeneration. In this study, we developed a thermosensitive hydrogel loaded with dithiothreitol (DTT) and stromal cell-derived factor-1 (SDF-1) and explored its effect on reshaping osteoblasts activity and facilitating periodontal bone regeneration. In vitro, the hydrogel [email protected] significantly reduced cellular ROS levels, reactivated the Wnt/β-catenin pathway of osteoblasts and restored their osteogenic ability in an inflammatory state. In vivo, the hydrogel [email protected] was applied in rat periodontitis models and showed satisfactory effects on reducing the level of ROS damage, reactivating the Wnt/β-catenin pathway, alleviating the inflammation level, and promoting the periodontal bone regeneration, simultaneously. Taken together, these results suggest that the combined application of DTT and SDF-1 facilitates in situ periodontal tissue regeneration and the thermosensitive hydrogel [email protected] is a promising candidate for periodontal therapy.
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