巨噬细胞
炎症
表型
外周血单个核细胞
免疫学
脂肪组织
集落刺激因子
生物
粒细胞巨噬细胞集落刺激因子
病理
细胞生物学
细胞因子
医学
体外
干细胞
内分泌学
造血
生物化学
基因
作者
Francisco Ríos,Rhian M. Touyz,Augusto C Montezano
出处
期刊:Methods in molecular biology
日期:2017-01-01
卷期号:: 311-320
被引量:22
标识
DOI:10.1007/978-1-4939-6625-7_24
摘要
Macrophage subtypes display protective or pathogenic activities in vascular lesions. They recognize and engulf modified lipids, accumulate in the arterial intima, contributing to the atherosclerosis development. In the heart, vascular tissues and perivascular adipose tissues, there is increasing evidence that macrophages play a role in endothelial dysfunction, vascular inflammation and remodeling. In this chapter we describe protocols to isolate human monocytes from peripheral blood mononuclear cell and how to differentiate them into macrophages by using growth factors (granulocyte-macrophage colony-stimulating factor-GM-CSF or colony-stimulating factor-M-CSF) or by different cell culture medium concentration. We also describe how to indentify different macrophage activation phenotypes.
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