甘薯
转录因子
拟南芥
激活剂(遗传学)
基因
侧根
生物
电泳迁移率测定
细胞生物学
拟南芥
发起人
植物
化学
生物化学
基因表达
突变体
作者
Xiaofeng Bian,Ho Soo Kim,Sang‐Soo Kwak,Qian Zhang,Shuai Liu,Pengda Ma,Zhang Jia,Yingxin Xie,Peng Zhang,Yang Yu
标识
DOI:10.3389/fpls.2022.820450
摘要
Plant root systems are essential for the uptake of water and nutrients from soil and are positively correlated to yield in many crops including the sweetpotato, Ipomoea batatas (L.) Lam. Here, we isolated and functionally characterized IbRAP2.4, a novel nuclear-localized gene encoding the AP2/ERF transcription factor, from sweetpotato. IbRAP2.4 was responsive to NaCl, PEG8000, ethylene, and Indole 3-acetic acid treatments. As revealed by electrophoretic mobility shift assay and dual luciferase assay, IbRAP2.4 could bind to both DRE and GCC-box elements and acted as a transcription activator. IbRAP2.4 overexpression significantly promoted lateral root formation and enhanced the drought tolerance in Arabidopsis thaliana, while it inhibited storage root formation in transgenic sweetpotato by comprehensively upregulating lignin biosynthesis pathway genes. Results suggested that IbRAP2.4 may be a useful potential target for further molecular breeding of high yielding sweetpotato.
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