EFFECTS OF SOME PICORNAVIRUS INHIBITORS ON THE REPLICATION OF FELINE CALICIVIRUS FCV IN CRFK CELLS

Obtaining of substances suppressing replication of caliciviruses is of special interest due to their particular role in the veterinary and human infectious pathology. Investigations for development of effective anti-calicivirus chemotherapy are important due to lack of specific means for calicivirus infections treatment. Caliciviridae possess a RNA(+) genome and similar structure as Picornaviridae, but reveal a different genome strategy. In a view of some similarities, a screening for anti-calicivirus activity of several highly efficient inhibitors of picornavirus replication was carried out. Research was carried out with Feline calicivirus (FCV), Crandell’s Feline Kidney cell line (CrFK) and the following compounds: Arildone, Disoxaril, S-7, Guanidine hydrochloride, PTU-23, HBB, Ribavirin and Oxoglaucine. Anti-calicivirus activity and citotoxicity were tested through CPE inhibition test and neutral red uptake assay (vs. virus inoculating doses ranging within 1 and 10 000 CCID50). Significant effects of HBB, PTU-23, Ribavirin and Oxoglaucine and a slight activity of S-7 were indicated, while Arildone, Disoxaril and Guanidine hydrochloride did not show influence.