豆科根瘤菌
突变体
根瘤菌
化学
生物化学
根瘤菌科
生物
细菌
基因
遗传学
共生
作者
H.C.J. Canter Cremers,Michael Batley,John W. Redmond,L Eydems,M.W. Breedveld,L P Zevehuizen,E. Pees,C. A. Wijffelman,Ben Lugtenberg
标识
DOI:10.1016/s0021-9258(17)45335-x
摘要
Rhizobium leguminosarum bv. viciae Exo- mutant strains RBL5523,exo7::Tn5,RBL5523,exo8::Tn5 and RBL5523,exo52::Tn5 are affected in nodulation and in the syntheses of lipopolysaccharide, capsular polysaccharide, and exocellular polysaccharide. These mutants were complemented for nodulation and for the syntheses of these polysaccharides by plasmid pMP2603. The gene in which these mutants are defective is functionally homologous to the exoB gene of Rhizobium meliloti. The repeating unit of the residual amounts of EPS still made by the exoB mutants of R. leguminosarum bv. viciae lacks galactose and the substituents attached to it. The R. leguminosarum bv. viciae and R. meliloti exoB mutants fail to synthesize active UDP-glucose 4'-epimerase.
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