Graphene-Based Glucose Sensors with an Attomolar Limit of Detection

footprint, ensuring high device uniformity while enabling detection in 40 μL analyte volume. A comprehensive suite of techniques─including Raman spectroscopy, X-ray photoelectron spectroscopy, and water contact angle measurements─reveals the stepwise evolution of graphene chemistry and surface properties leading to the controlled immobilization of glucose oxidase. Our findings demonstrate p-type doping and tensile strain in the graphene channel across the nanomolar-millimolar glucose concentration range. The enzyme-catalyzed oxidation of glucose produces hydrogen peroxide in close proximity to the graphene channel, inducing a systematic shift in the Dirac point voltage toward more positive values. Under these conditions, the biosensor achieves an attomolar limit of detection and a sensitivity of 10.6 mV/decade, outperforming previously reported glucose sensors. Selectivity tests against common interferents such as lactate and ascorbic acid, as well as validation in artificial and human tears, demonstrate its robustness for real-world applications. Altogether, these findings position the electrolyte-gated graphene field-effect transistor as a transformative, noninvasive glucose-sensing platform, paving the way for next-generation continuous monitoring devices, including wearable formats for real-time, user-friendly diabetes management.