Multi-residue pesticides determination in complex food matrices by gas chromatography tandem mass spectrometry

An analytical method for determination of GC-amenable pesticides in complex matrices, was validated based on QuEChERS sample preparation and gas chromatography tandem mass spectrometry. Performance of the method was tested according to the EU SANTE guidelines for 172 pesticides belonging to different chemical classes, in three representative complex matrices. Three concentration levels were tested in order to establish the lowest limit of quantification possible. For some matrix/pesticide combination, careful selection of the quantification/confirmation transitions was key to avoid interferences. Accurate quantification was achieved by standard addition. The number of compounds fulfilling EU SANTE criteria at 10 µg/kg and 100 µg/kg were 93 and 148 for roasted coffee, 93 and 112 for green tea and 98 and 111 for curry respectively. The method was further evaluated in different matrices (chili, clove, cumin, paprika and rosemary) with 50-90 % of the compounds fulfilling the validation criteria depending on the matrix.