环介导等温扩增
DNA提取
生物
病菌
滤波器(信号处理)
萃取(化学)
DNA
基因组DNA
聚合酶链反应
灵敏度(控制系统)
园艺
材料科学
植物
色谱法
微生物学
基因
遗传学
计算机科学
化学
电子工程
计算机视觉
工程类
作者
Huayan Zou,Taiwen Li,Jing Zhang,Hu Shao,Koji Kageyama,Wenzhuo Feng
出处
期刊:Plant Disease
[Scientific Societies]
日期:2024-01-01
卷期号:108 (1): 35-40
被引量:1
标识
DOI:10.1094/pdis-05-23-0913-sc
摘要
The pathogen Colletotrichum siamense causes tea anthracnose, resulting in economic losses to the Chinese tea industry. To effectively diagnose this pathogen in the field, we developed a loop-mediated isothermal amplification (LAMP) method using highly specific primers with a sensitivity of 1 pg/μl designed for amplifying the CAL gene, which was 10 times higher than that of conventional PCR. Additionally, to improve the method for obtaining DNA samples required for on-site diagnosis, we used the filter-disc DNA extraction method, which does not require special instruments and can be completed in a few minutes, and found that it effectively meets the requirements for the LAMP reaction. Finally, we combined LAMP with a filter-disc DNA extraction method (FDE-LAMP) to diagnose different degrees of disease in inoculated samples and 20 samples from the field. The results showed that the procedure had sufficient sensitivity for pathogen detection. Therefore, the FDE-LAMP procedure could greatly contribute to managing and preventing tea anthracnose in the field.
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