Fluorescence sensing platform for Cronobacter sakazakii based on the cationic metal-organic frameworks modified upconversion nanoparticles

Cronobacter sakazakii (C. sakazakii) is a serious threat to human health. Efficient detection of C. sakazakii is crucial for food safety and the control of foodborne diseases. Here, a fluorescence sensing platform for C. sakazakii was constructed with α-glucosidase and cationic metal-organic framework-functionalized upconversion nanoparticles ([email protected]). The anionic α-glucosidase was inhibited by binding to cationic [email protected] The presence of C. sakazakii competitively combined with cationic [email protected] and released α-glucosidase into the enzyme substrate solution (para-nitrophenyl-α-d-glucopyranoside) to recover activity, thus triggering fluorescence quenching of cationic [email protected] through the product of 4-nitrophenol. In addition, α-glucosidase from C. sakazakii accelerated the formation of 4-nitrophenol, thus improving the fluorescence response of cationic [email protected] This strategy illustrates high sensitivity of the designed platform for C. sakazakii with a wide linear range (1.1 × 102–1.1 × 108 cfu mL−1), a low limit of detection (3.2 × 101 cfu mL−1), and an acceptable recovery value (85.2–111.5%) in real samples (tap water, infant formula powder, and goat milk powder). These results confirm that the proposed fluorescence platform is an ideal candidate tool for the detection of C. sakazakii in microbiological diagnostics.