Recent Advances and Optimization Strategies in Chemiluminescence for Enhanced Bioimaging Performance†

Comprehensive Summary Known for high sensitivity and significant signal‐to‐noise ratio, chemiluminescence (CL), a phenomenon dependent on chemiexcitation via chemical reaction, has gained much attention in the field of in vitro detection, biosensing, and in vivo imaging over the past few years. Albeit its fulminant development, bottlenecks, such as poor stability, limited structural tunability, low emission intensity, and uncontrollable reaction rates restrict biological applications and further practical translation. Therefore, researchers have been dedicated to improving CL performance for broader and meaningful programs. This review first provides a concise outline of the basic concepts of CL, luminescence mechanisms, and development history, then summarizes key optimization strategies focused on three main areas for improving imaging performance: extending the emission wavelength, enhancing luminescent properties, and improving synthetic feasibility, followed by ending with a discussion of emerging opportunities and future directions in chemiluminescence research.