替罗非班
医学
血小板
纤维蛋白
凝结
纤溶
水蛭素
弥漫性血管内凝血
免疫学
肺
内科学
H&E染色
输血相关性急性肺损伤
胃肠病学
病理
肺水肿
凝血酶
免疫组织化学
传统PCI
心肌梗塞
作者
Xin Yuan,Peng Jiang,CongChao Qiao,Naichuan Su,Pingping Sun,Faquan Lin,Changqing Li
出处
期刊:Shock
[Ovid Technologies (Wolters Kluwer)]
日期:2023-01-14
卷期号:59 (4): 603-611
被引量:1
标识
DOI:10.1097/shk.0000000000002080
摘要
This study aimed to explore the ameliorating effects of the platelet surface glycoprotein IIb/IIIa receptor antagonist tirofiban on coagulation and fibrinolytic abnormalities in a mouse model of antibody-mediated transfusion-associated acute lung injury (ALI). This is important because ALI is a major cause of death attributable to the occurrence of adverse transfusion reactions. No information on a definite diagnosis or pathological mechanism exists, and targeted treatment options are not available. In this study, wild-type male Balb/c mice aged 8 to 10 weeks were randomly divided into the TRALI model, blank control, tirofiban intervention, and isotype control groups. After different treatment exposures, the mice were observed for 2 h before being killed, and lung tissue samples were collected. To explore the intervention effect of tirofiban, the degree of lung injury was quantified by estimating the lung wet/dry ratio, rectal temperature, survival rate, total protein, and myeloperoxidase and via hematoxylin-eosin staining. Furthermore, the coagulation, anticoagulation, and fibrinolysis assays were measured by automatic coagulation instrument and enzyme-linked immunosorbent assay kits, and the fluorescence densities of platelets and fibrin were quantified using immunofluorescence to analyze the effects of tirofiban on the platelet and fibrin interactions of TRALI. Compared with the TRALI model group, the lung injury indices in the tirofiban intervention group decreased significantly, and survival rates also improved. Furthermore, the level of coagulation and fibrinolytic abnormalities were obviously lower than those in the TRALI model group. In conclusion, our findings suggest that tirofiban might interfere with TRALI by inhibiting platelet activation and improving coagulation and fibrinolytic abnormalities.
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