三七
小胶质细胞
神经发生
冰片
神经炎症
TLR4型
化学
药理学
槽口1
神经保护
医学
细胞生物学
炎症
生物
生物化学
内科学
信号转导
Notch信号通路
病理
替代医学
中医药
作者
Huang Ding,Xiaoping Huang,Xiaodan Liu,Yanling Li,San Tang,Hai-Long Xiong,Mei-Ting Huang,Ying Liu,Caixia Liu,Wei Zhang,Chang-Qing Deng
摘要
Abstract Objective To study the effect of borneol combined with astragaloside IV and Panax notoginseng saponins (BAP) on promoting neurogenesis by regulating microglia polarization after cerebral ischaemia–reperfusion(CI/R) in rats. Methods A focal CI/R injury model was established. Evaluated the effects of BAP on ischaemic brain injury, on promoting neurogenesis, on inhibiting Inflammatory microenvironment and TLR4/MyD88/NFκB signalling pathway. A microglia oxygen-glucose deprivation reoxygenation (OGD/R) model was established that evaluated the effects of BAP on regulating the polarization of microglia and inflammatory microenvironment. Results BAP can inhibit the expression of TLR4, MyD88 and NFκB proteins, reduce IL-1β and increase IL-10, reduce M1 type microglia and increase M2 microglia. The proliferation of neural stem cells increased, synaptic gap decreased, synaptic interface curvature increased, expression of SYN and PSD95 proteins increased, which improved the neurological dysfunction and reduced the volume of cerebellar infarction and nerve cell injury. Conclusion BAP can reduce CI/R injury and promote neurogenesis, the effect is related to inhibition of the activation of TLR4/MyD88/NFκB, regulating the polarization of microglia from M1 type to M2 type and inhibition of inflammatory response.
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